CHE 626

THE UNIVERSITY OF KENTUCKY

DEPARTMENT OF CHEMISTRY

CHE 626 Advanced Analytical Chemistry

 


Course Description: An advanced study of the theory and practice of quantitative analysis.

Course Information

Academic Dishonesty

Academic dishonesty or cheating of any kind will not be tolerated. This is a serious offense and the instructor will make every effort to ensure that the punishment is immediate and severe. This is also the official policy of the Department of Chemistry.


Typical Course Syllabus, CHE 626

I.  Introductory Topics
     A.  Development of Analytical Chemistry
     B.  Analytical Terms
     C.  Precision and Accuracy
     D.  Figures of Merit

II.  Measurement Fundamentals
     A.  Signal-to-Noise Ratio
     B.  Origin of Instrument Noise
     C.  Devices for S/N Enhancement
     D.  Software-Based Methods for S/N Enhancement
     E.  Quantifying Measurements and Extracting Information

III.  Automated Methods of Analysis
     A.  Need for Automated Methods
     B.  PC-Based Data Acquisition
     C.  Continuous Flow Analysis
     D.  Flow-Injection Analysis
     E.  Multilayer Dry Reagent Slides
     F.  Laboratory Robots

IV.  Chemical Equilibria in Modern Analytical Chemistry
     Binding Assays/Immunoassays

V.  Introduction to Spectroscopy
     A.  Electromagnetic Radiation
     B.  Instrument Components
     C.  Sources of Radiation
     D.  Wavelength Selectors
     E.  Detectors

VI.  Atomic Spectroscopy
     A.  Principles
     B.  Flame Emission Spectroscopy
     C.  Inductively Coupled Plasma
     D.  Flame Atomic Absorption Spectroscopy
     E.  Graphite Furnace Atomic Absorption Spectroscopy
     F.  Background Correction in Atomic Absorption Spectroscopy
     G.  Atomic Fluorescence
     H.  X-Ray Fluorescence

VII.  Molecular Spectroscopy
     A.  UV-Visible Spectroscopy
     B.  Molecular Luminescence
     C.  Infrared Spectroscopy
           Raman

VIII.  Introduction to Chromatographic Separations
     A.  Classification
     B.  Chromatographic Parameters
     C.  Resolution
     D.  Kinetic Variables Affecting Band Broadening

IX.  Liquid Chromatography
     A.  HPLC Instrumentation
     B.  Adsorption Chromatography
     C.  Partition Chromatography
     D.  Other Types of Liquid Chromatography

X.  Gas Chromatography
     A.  Basic Description
     B.  Classification of GC Methods
     C.   Stationary Phase
     D.  Carrier Gas
     E.  Detectors
     F.  Temperature Programming
     G.  Hyphenated Methods

XI.  Introduction to Electroanalytical Chemistry
     A.  Concepts, Terms and Symbols
     B.  Types of Electrodes
     C.  Liquid Junction Potential
     D.  Types of Electrochemical Cells
     E.  Cell Potential
     F.  Nernst Equation

Bibliography

I. Introductory Topics
  1. Skoog and Leary, Chapter 1 and Appendix 1
  2. Majors, LC.GC, 10, 500-506 (1992)
  3. Horwitz, Pure Appl. Chem., 62, 1193-1208 (1990)
  4. Gy, LC.GC, 12, 808-814 (1994)
II. Measurement Fundamentals
  1. Skoog and Leary, Chapter 4
  2. Coor, J. Chem. Educ., 45, A533-A544 (1968)
  3. Coor, J. Chem. Educ., 45, A583-A596 (1968)
  4. Hieftje, Anal. Chem., 44, 81A-88A (1972)
  5. Savitzky and Golay, Anal. Chem., 36, 1627-1630 (1964)
III. Automated Methods of Analysis
  1. Skoog and Leary, Chapter 28
  2. Dehne, Anal. Chem., 62, 565A-572A (1990)
  3. van der Linden, Pure Appl. Chem., 66, 2493-2500 (1994)
  4. Fraticelli and Meyerhoff, Anal. Chem., 55, 359-364 (1983)
  5. Andrew et al., Anal. Chem., 66, 916A-922A (1994)
  6. Sjodin and Sundqvist, Am. Lab., January 1989, 63-67
  7. Newman, Anal. Chem., 62, 29A-34A (1990)
  8. Felder et al., Anal. Chem., 63, 741A-747A (1991)
IV. Chemical Equilibria in Modern Analytical Chemistry
  1. Juaristi, J. Chem. Educ., 60, 721-724 (1983)
  2. Nakamura and Kasahara, in "Immunochemical Assays and Biosensor
  3. Technology for the 1990's", 1992
  4. Monroe, Anal. Chem., 56, 920A-931A (1984)
  5. Skoog, West, and Holler, Analytical Chemistry an Introduction, 6th Ed., 132-135
V. Introduction to Spectroscopy
  1. Skoog and Leary, Chapters 5 and 6, and page 212 for hollow-cathode lamp
  2. Coleman, J. Chem. Educ., 59, 441-445 (1982)
  3. Wohlstein, Spectroscopy, 4, 10-15 (1989)
VI. Atomic Spectroscopy
  1. Skoog and Leary, Chapters 10 and 11
  2. Skoog and Leary, Chapter 15A-3 and 15C for X-Ray Fluorescence
  3. Noble, Anal. Chem., 66, 105A-109A (1994)
  4. Beauchemin, Spectroscopy, 7(7), 12-18 (1992)
  5. Meyer, Anal. Chem., 59, 1345A-1354A (1987)
  6. Olesik, Anal. Chem., 63, 12A-21A (1991)
  7. Miller-Ihli, Anal. Chem., 64, 964A-968A (1992)
  8. Wach, Anal. Chem., 67, 51A-55A (1995)
  9. Brown, Anal. Chem., 49, 126A-128A (1977)
VII. Molecular Spectroscopy
  1. Skoog and Leary, Chapters 7, 8, and 9
  2. Skoog and Leary, Chapter 6G for Fiber Optics
  3. Anderson et al., IEEE Eng. Med. Biol., 1994, 358-363 (1994)
  4. Skoog and Leary, Chapters 12 and 13 for IR and Raman
  5. Perkins, J. Chem. Educ., 63, A5-A10 (1986)
  6. Perkins, J. Chem. Educ., 64, A269-271 (1987)
VIII. Introduction to Chromatographic Separations
  1. Skoog and Leary, Chapter 24
  2. Ettre, Pure Appl. Chem., 65, 819-879 (1993)
  3. Hawkes, J. Chem. Educ., 60, 393 (1983)
IX. Liquid Chromatography
  1. Skoog and Leary, Chapter 26
X. Gas Chromatography
  1. Skoog and Leary, Chapter 25
XI. Introduction to Electroanalytical Chemistry
  1. Skoog and Leary, Chapter 19

Additional Literature

I.    Kolthoff, Anal. Chem., 45, 24A-37A (1973)
      Greenaway, Anal. Chem., 48, 148A-154A (1976)
      Hieftje, Am. Lab., October 1993, 53-61
      Malissa, Fresenius' Z. Anal. Chem., 319, 357-363 (1984)

III.  Clark et al., CRC Crit. Rev. Anal. Chem., 21, 357-375 (1990)

IV.   Portsmann and Kiessig, J. Immunol. Methods, 150, 5-21 (1992)
      Cosling, Clin. Chem., 36, 1408-1427 (1990)
      Yallow and Berson, Nature, 184, 1648-1649 (1959)

VI.   Falk, CRC Crit. Rev. Anal. Chem., 19, 29-64 (1988)
      Tūlg, Fresenius' Z. Anal. Chem., 331, 226-235 (1988)

VII.  McClure, Anal. Chem., 66, 43A-53A (1994)

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