Bert C. Lynn

Professor of Chemistry
Director, UK Mass Spectrometry Facility
Analytical Chemistry
Proteomics

A053 ASTeCC Building
Phone: (859) 2187-6529
Fax: (859) 257-2489
Email: bclynn2@uky.edu

1979 B.S., University of Mississippi.
1987 Ph.D., Mississippi State University.

Mass spectrometry research in my group is divided into three broad areas: 1) proteomics and biological mass spectrometry, 2) environmental methods development working at the interface between environmental chemistry and biology and 3) quadrupole ion trap instrument development.

1 - Proteomics projects include development of biomarkers in CSF of Alzheimer's Disease (AD), determination of the proteome of adult stem cells and neuronal mitochondria, transcription of MnSOD during tumorgenesis and determination of protein modifications in thermophilic anaerobic bacteria adapted to high ethanol environments.

2 - Environmental methods development is currently focused on chemical alterations in DNA (DNA adducts) caused by oxidative stress. Additionally, we are working on methods to determine aldehyde exposure and markers for exposure to environmental estrogen mimics and markers of exposure to environmental contaminates. We have recently developed methods to observe phosphorylation of the enzyme butyryl cholinesterase by organophosphorus insecticides. Example spectra: MALDI spectrum of the active site peptide, MALDI spectrum of the inhibited active site peptide, Capillary HPLC chromatogram of the inhibited active site peptide, and MS/MS spectrum of the inhibited active site peptide

3 - A variety of quadrupole ion trap (QIT) projects are underway including development of miniature cylindrical ion traps, ion tomography experiments, ion mobility in a QIT and alternative scan functions.

Nelson et al. "An Integrated Approach for Automating Validation of Extracted Ion Chromatographic Peaks" supplemental data