Developing
New and Useful RNA Catalysts.We have developed novel RNA catalysts, based on autocatalytic RNA group
I introns, which can recombine RNA in predetermined ways. Our most advanced,
the trans excision-spicing ribozyme, can excise out
single nucleotide insertion mutations from transcripts inside bacterial cells. In
this way, we have sequence-specifically repaired mutations in functional RNA
transcripts. Questions that remain, with this and other recently developed
catalysts, include how to develop them for optimal cellular activity, how they
fold and function, how they can be exploited for the development of new
biotechnology, and we then take these lessons to develop other useful
catalysts. Methods used in these projects involve molecular biology (for
example, PCR, cloning, site-directed mutagenesis, and gel electrophoresis).
Z-helical
hairpins.We have found a novel DNA hairpin that adopts a Z-helical
conformation as a function of the hairpin loop sequence (the DNA double helix
is left-handed instead of the usual right-handed). Somehow, the structure of
the loop is driving the stem into this unusual conformation. Therefore, we are
working toward fully understanding the sequence and structure requirements of
this molecule. Methods used in this project involve traditional biochemical
techniques (for example, UV spectroscopy, CD spectroscopy, and in the very near
future, NMR).
Thermodynamics.
We are working on a new project whereby we ascertain the effects of foreign
molecules on the structure and function of nucleic acids. Areas of interest include the effects of
cigarette smoke components on the stability of DNA and RNA duplexes. Methods
used in this project involve UV spectroscopy (specifically thermal denaturation analysis). These types of projects are
especially well suited for undergraduates, as well as graduate students who
desire to become educators at liberal arts colleges.
Scientific
Programming. I am working on a new project that combines my interest
in programming (using Microsoft Visual Studio 2005 – Visual Basic) with my
knowledge of nucleic acids and molecular biology. The first program is a
general purpose laboratory tool called STcalc, and
the second project I am about to begin focuses on molecular biology and
education.
See Also My Personal Web Page
Meet the
Members of the Testa Lab
Testa Lab Publications
Former Lab Ph.D's
Former Lab Masters' Students
