Testa Lab Publications

  • Dotson II, P. P., Sinha, J., and Testa, S. M. (2008) “A Pneumocystis carinii group I intron-derived ribozyme utilizes an endogenous guanosine as the first reaction step nucleophile in the trans excision-splicing reaction”, Biochemistry 47, 4780-4787.
  • Dotson II, P. P., Sinha, J., and Testa, S. M. (2008) Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction, The FEBS Journal, published online May 08, 2008.
  • Dotson II, P. P. & Testa, S. M. (2006) "Group I Intron-Derived Ribozyme Recombination Reactions" Recent Developments in Nucleic Acids Research, 2(2006): 307-324 ISBN: 81-7895-192-4.
  • Johnson, A. K., Sinha, J., & Testa, S. M. (2005) "Ribozyme-Catalyzed Insertion of Targeted Sequences into RNAs", Biochemistry 44, 10702-10710.

  • Baum, D. A. & Testa, S. M. (2005) "In Vivo Excision of a Single Targeted Nucleotide from an mRNA by a Trans Excision-Splicing Ribozyme", RNA 11, 897-905.

  • Alexander, R. C., Baum, D. A., & Testa, S. M. (2005) "5' Transcript Replacement in vitro Catalyzed by a Group I Intron-Derived Ribozyme", Biochemistry 44, 7796-7804.

  • Baum, D. A., Sinha, J., & Testa, S. M. (2005) "Molecular Recognition in a Trans Excision-Splicing Ribozyme: Non-Watson-Crick Base Pairs at the 5' Splice Site and wG at the 3' Splice Site Can Play a Role in Determining the Binding Register of Reaction Substrates", Biochemistry 44, 1067-1077.

  • Bell, M. A., Sinha, J., Johnson, A. K., & Testa, S. M. (2004) "Enhancing the Second Step of the Trans Excision-Splicing Reaction of a Group I Ribozyme by Exploiting P9.0 and P10 for Intermolecular Recognition", Biochemistry 43, 4323-4331.

  • Alexander, R. C., Johnson, A. K., Thorpe, J. A., Gevedon, T., & Testa, S. M. (2003) "Canonical Nucleosides Can Be Utilized by T4 DNA Ligase as Universal Template Bases at Ligation Junctions", Nucleic Acids Res. 31, 3208-3216.

  • Johnson, A. K., Baum, D. A., Tye, J., Bell, M. A., & Testa, S. M. (2003) "Molecular Recognition Properties of IGS-Mediated Reactions Catalyzed by a Pneumocystis carinii Group I Intron", Nucleic Acids Res. 31, 1921-1934.

  • Bell, M. A., Johnson, A. K., & Testa, S. M. (2002) "Ribozyme-Catalyzed Excision of Targeted Sequences from within RNAs", Biochemistry 41, 15327-15333.

  • Disney, M. D., Testa, S. M., & Turner, D. H. (2000) "Targeting a Pneumocystis carinii Group I Intron with Methylphosphonate Oligonucleotides: Backbone Charge is Not Required for Binding or Reactivity", Biochemistry 39, 6991-7000.
     
  • Testa, S. M., Turner, D. H., & Kierzek, R. (1999) "Thermodynamics of RNA-RNA Duplexes with 2- or 4-Thiouridines: Implications for Antisense Design and Targeting a Group I Intron", Biochemistry 38, 16655-16662.
     
  • Testa, S. M, Gryaznov,  S. M., & Turner, D. H. (1999) "In Vitro Suicide inhibition of self-splicing of a group I intron from Pneumocystis carinii by an N3'->P5' phosphoramidate hexanucleotide", Proc. Natl. Acad. Sci. U.S.A. 96, 2734-2739.
  • Testa, S. M., Gryaznov,  S. M., & Turner, D. H. (1998) "Antisense Binding Enhanced by Tertiary Interactions: Binding of Phosphorothioate and N3'-> P5' Phosphoramidate Hexanucleotides to the Catalytic Core of a Group I Ribozyme from the Mammalian Pathogen Pneumocystis carinii", Biochemistry 37, 9379-9385.
  • Testa, S. M. , Haidaris, C. G., Gigliotti, F., & Turner, D. H. (1997) "A Pneumocystis carinii Group I Intron Ribozyme that Does Not Require 2' OH Groups on its 5' Exon Mimic for Binding to the Catalytic Core", Biochemistry 36, 15303-15314.

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  • Profenno, L., Kierzek, R., Testa, S. M., & Turner, D. H. (1997) "Guanosine Binds to the Tetrahymena Ribozyme in More than One Step, and Its 2' OH and the Nonbridging pro-Sp Phosphoryl Oxygen at the Cleavage Site Are Required for Productive Docking", Biochemistry 36, 12477-12485.

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  • Testa, S. M, & Gilham, P. T. (1993) "Analysis of Oligonucleotide Structure using Hyperchromism Measurements at Long Wavelengths", Nucleic Acids Res. 21, 3907-3908.