Nuclear Magnetic Resonance Facility

University of Kentucky, Department of Chemistry

Room 33NMR, Chemistry-Physics Building

(859) 257-1183

 

Skills on NMR is becoming more and more important and is required for almost all the academic and industrial jobs.

To learn more about NMR, please pick up the Advanced NMR course conducted by Dr. Miller.

Click here to get the comprehensive NMR manual written by Dr. Miller.

Welcome to the UK Chemistry NMR Facility

The facility currently comprises 6 instruments, with one more being installed right now. In addition, there are several data stations for offline processing of NMR data. Specific instruments are:

Graduate students get hands-on experience on NMR

Two Varian Gemini 200 MHz NMR Spectrometers

The NMR center provides two upgraded GEMINI 200 MHz instruments. One is a dedicated H/C instrument, the other has multinuclear and variable temperature capability. These instruments are used for the routine characterization of organic and organometallic materials. You may reserve blocks of NMR time in advance or you may use the instruments as a walk-on user if no one has reserved the instruments. Obtaining NMR time is not a problem in this department.

 

Postdocs and Research associates are being extensively trained in running NMR spectrometers.

Two Varian INOVA 400's

These 400 MHz instruments (one purchased in 1999, one upgraded in 1999) have a z-pulsed field gradients (PFG), variable temperature capabilities, waveform generators, and can perform all of the latest 2- and 3-dimensional NMR experiments (COSY, NOESY, ROESY, TOCSY, DEPT, INADEQUATE, and other advanced NMR pulse sequences). Observation of any NMR-active nucleus in the periodic table is possible. These are walk-on instruments (or reserve time if you've got a long experiment to run) for use by students, faculty, and postdocs, but our staff spectroscopist John Layton can run your sample for you if needed.

John is always ready to help you shoot problems that may arise at any time.

 

Varian INOVA 600

This new (installed in 2000) instrument is equipped with 4 rf channels, 40 room temperature shims, z-PFG, triple resonance and broadband switchable probes, the works! Specifically configured for the n-dimensional NMR study of large biological molecules, this is a state-of-the-art machine that pushes the limit of instrument technology.

Want to know more about this instrument? Contact Professor Anne-Frances Miller, the major user of this top-flight machine. In the picture below she is programming a new pulse sequence for use in her research on superoxide dismutase.

Dr. Miller is teaching Peng Zhang to run 3D protein NMR

 

Varian INOVA 400wb Solids NMR Spectrometer

This instrument, funded by an NSF-MRI grant, brings to the Department the ability to study samples in the solid state. The instrument has a full solids package, including the capability to do CP-MAS, REDOR, wideline, and multipulse experiments. This instrument can spin samples at 15 kHz and has kilowatt amplifiers on both rf channels.

Peng Zhang is processing 3D NMR data.

Who's Who in the NMR Lab

 

Day to day operations of the NMR facility are overseen by full time spectroscopist, John Layton, wjl@pop.uky.edu, (859) 257-1183. John can usually be found in the NMR lab or in his office in room 7 of the Chemistry-Physics Building. John maintains the instruments and provides individual instruction so that students who are beginning their research can learn to operate all of the NMR spectrometers in a matter of weeks.

 

 

Useful Info and Tips

The Toreki group has prepared some handy instructions sheets for the Gemini 200 broadband instrument. These are in PDF format:

In addition, CHE 450G has an HTML page explaining how to run a basic proton NMR. It's a good review/intro sheet for new users.

 

[Departmental Resources]   [UK Chemistry Home Page]

This page was last updated by Cungen Zhang on August 12, 2005